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This entry is from Winter semester 2022/23 and might be obsolete. You can find a current equivalent here.

Introduction to Drosophila Genetics
(dt. Einführung in die Drosophila-Kreuzungsgenetik)

Level, degree of commitment Profile module, compulsory elective module
Forms of teaching and learning,
workload		 Recitation class “Einführung in die Genetik von Drosophila me,
180 hours (recitation class: 30 h private study inkl. Vorbereitung and Ablegen der Prüfungen: 150 h)
Credit points,
formal requirements		 6 CP
Course requirement(s):
Examination type: Protocol
Language,
Grading		 German,
The grading is done with 0 to 15 points according to the examination regulations for the degree program B.Sc. Biologie.
Duration,
frequency		 One semester,
each winter semester
Person in charge of the module's outline Buttgereit, N.N. (V)

Contents

Determination of simple heritabilities using maize (Zea mays) as an example, basics of Drosophila genetics; chromosome number, balancers; the P element and other transposons in Drosophila; specific application of the P element: the GAL4/UAS system; further modifications: Recombination using FRT/FLP; targeted generation of deficiencies for individual genes (phi/Cre/Lox).

Independent performance of experiments and acquisition of knowledge on Drosophila strain maintenance, male/female differentiation, fly handling; identification and recognition of balancer strains based on phenotypic markers; set-up and analyses of crossing plans; Determination of chromosomal localization of transgenes; Crossing with balancer strains; Jump-out experiments to produce mutants of single genes; FRT/FLP crosses to generate genomic deficiencies; Meiotic recombination to generate double mutants, resp. Labeling of chromosomes using multimarker techniques; GAL4-UAS crosses for analysis of mutant phenotypes, evaluation of adult structures (eye, wing, etc.); analysis of mutants using in situ hybridization/antibody staining.


Qualification Goals

Students should acquire basic knowledge of Drosophila genetics that goes well beyond the knowledge of classical genetics. In particular, the targeted use of transposons for the analysis and generation of specific mutants will be taught in this module. Here, in addition to the theoretical basics, independent practical skills in the design and execution of crossing experiments will be taught in particular, as well as the detailed presentation of a crossing plan and the critical evaluation of the data.


Prerequisites

None.


Applicability

Module imported from B.Sc. Biologie.

When studying M.Sc. Mathematics, this module can be attended in the study area Minor subject Biology.


Recommended Reading

(not specified)



Please note:

This page describes a module according to the latest valid module guide in Winter semester 2022/23. Most rules valid for a module are not covered by the examination regulations and can therefore be updated on a semesterly basis. The following versions are available in the online module guide:

The module guide contains all modules, independent of the current event offer. Please compare the current course catalogue in Marvin.

The information in this online module guide was created automatically. Legally binding is only the information in the examination regulations (Prüfungsordnung). If you notice any discrepancies or errors, we would be grateful for any advice.